News
09 October 2024
Road to Research: A Q&A with Laboratory Manager, Seth Malloy
"Every day presents a new challenge, a new question, and a new opportunity to positively impact the research community."
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Histology
The Histology team provides histotechnical instrumentation, technical support, and collaborative services to Stowers Institute scientists whose research involves the microscopic anatomy of biological tissues and cells.
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Publications
Overview of Services
Research Services
Comprised of histotechnologists and biologists with backgrounds in cytogenetics, molecular biology and neuroscience, the Histology Team collaborates with Stowers colleagues on projects to understand the spatial composition of RNA, protein, or other components in various types of samples, ranging from chromosome spread, cells, and tissue sections to whole mount organisms. The team has earned a reputation for creativity in developing histological methods to enable scientific discoveries.
Technologies
The Stowers Histology center is a full-service histology laboratory that offers a variety of histological techniques to Institute members. We process fresh, paraffin-embedded and frozen tissue for routine histology, special stains, immunohistochemical assays, tissue clearing, in-situ hybridization, expansion microscopy, and spatial transcriptomics & proteomics. Additionally, we provide investigators with training, consultation and advice on how to best use histology services.
- LifeCanvas SmartClear II Pro and SmartLabel
- Intavis InsituPro Vsi robot
- HTXImaging M3+ Matrix Sprayer
- Thermo Cryostar NX70
- Thermo Cytospin4
- HybEZ-II hybridization oven
- Biogenex EZ-Retriever
- Milestone Pathos Delta processor
- Leica Autostainer XL and CV5030
- Leica EG1150 embedding center
- Leica cryoJane ECU500
- Bio-Rad C1000Touch Thermal cycler
- Leica VT1200 and VT1000S vibratome
- Leica HistoCore Autocut microtome
- Thermo Microm HM355S microtome
- Leica CM3050S cryostat
Team Contact
Kexi Yi
Director of Microscopy
Stowers Institute for Medical Research
Kexi Yi joined the Stowers Institute for Medical Research in 2009 as a research specialist in the lab of former Investigator Rong Li, Ph.D. He joined the Microscopy Technology Center in 2015 and was promoted to Head of Electron Microscopy in 2023.
Team Contact
Seth Malloy
Manager, Histology
Stowers Institute for Medical Research
Seth Malloy joined the Stowers Institute for Medical Research in the Histology Technology Center in 2018, and was promoted to manager of Histology in 2023.
News from the lab
Press Release
01 January 2021
A niche for the eye
What if degenerative eye conditions could be detected and treated before vision is impaired? Recent findings from the Xie Lab point to the ciliary body as a key to unlocking this possibility.
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Video
04 September 2020
Not your usual fish tale
A comparative study of two fish species offers important clues about vertebrate regeneration.
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Featured Publications
Pang J, Thomas N, Tsuchiya D, Parmely T, Yan D, Xie T, Wang Y. STAR Protoc. 2021;2:100879. doi: 100810.101016/j.xpro.102021.100879..
Changes in regeneration-responsive enhancers shape regenerative capacities in vertebrates
Wang W, Hu CK, Zeng A, Alegre D, Hu D, Gotting K, Ortega Granillo A, Wang Y, Robb S, Schnittker R, Zhang S, Alegre D, Li H, Ross E, Zhang N, Brunet A, Sanchez Alvarado A. Science. 2020;369: eaaz3090. doi: 10.1126/science.aaz3090.
Peuß R, Box AC, Chen S, Wang Y, Tsuchiya D, Persons JL, Kenzior A, Maldonado E, Krishnan J, Scharsack JP, Slaughter BD, Rohner N. Nat Ecol Evol. 2020;4:1416-1430.
Wang Y, Xu W, Maddera L, Tsuchiya D, Thomas N, Yu CR, Parmely T. J Histotechnol. 2019;42:193-201.
Wang Y, Yu Z, Cahoon CK, Parmely T, Thomas N, Unruh JR, Slaughter BD, Hawley RS. Nat Protoc. 2018;13:1869-1895.
Zeng A, Li H, Guo L, Gao X, McKinney S, Wang Y, Yu Z, Park J, Semerad C, Ross E, Cheng LC, Davies E, Lei K, Wang W, Perera A, Hall K, Peak A, Box A, Sánchez Alvarado A. Cell. 2018;173:1593-1608.e20.
